Summary
Pyruvate dehydrogenase complex activity and its regulating factors were measured in
diabetic mice in a starved or unstarved state. Both in starved and unstarved groups
of normal mice, pyruvate dehydrogenase complex activity increased equally by insulin
administration. On the other hand, in an unstarved group of diabetic mice, no change
in pyruvate dehydrogenase complex activity was observed with the same treatment. With
the starved group it increased within 10 minutes and recovered to the base value level
in 30 minutes. As a result of studies on active form of pyruvate dehydrogenase complex
in the liver and its regulating factors, it was found that no corresponding relation
existed between the enzyme complex activity and its regulating factors. We found that
unlike the normal mice, not only the previously known regulating mechanism but also
other factors affected the pyruvate dehydrogenase complex activity in the diabetic
mice.
Key-Words:
Pyruvate Dehydrogenase Complex
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Alloxan Diabetic Mouse
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Phosphorylation and Dephosphorylation Reaction
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Interconversion of Active and Inactive Forms
